Extracellular vesicles play important roles in physiological and pathological processes, including coagulation, immune responses, and cancer or as potential therapeutic agents in drug delivery or regenerative medicine. This protocol presents methods for the quantification and size characterization of isolated and non-isolated extracellular vesicles in various fluids using tunable resistive pulse sensing.
Maas, S. L. N., De Vrij, J., Broekman, M. L. D. Quantification and Size-profiling of Extracellular Vesicles Using Tunable Resistive Pulse Sensing. J. Vis. Exp. (92), e51623, doi:10.3791/51623 (2014).