ジャーナル
/
免疫学と感染
/
A Method for Generating Pulmonary Neutrophilia Using Aerosolized Lipopolysaccharide
JoVE Journal
免疫学と感染
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 免疫学と感染
A Method for Generating Pulmonary Neutrophilia Using Aerosolized Lipopolysaccharide

A Method for Generating Pulmonary Neutrophilia Using Aerosolized Lipopolysaccharide

11,539 Views

•

08:33 min

•

December 15, 2014

DOI:

10.3791/51470-v

DOI:
10.3791/51470-v

•

08:33 min
December 15, 2014

•

1 Views
, , , ,
1Department of Medicine, Solna and CMM, Respiratory Medicine Unit,Karolinska Institutet, 2Safety Science, Global Regulator Affairs & Patient Safety,AstraZeneca Global Medicines Development

概要

Automatically generated

We describe a method for inducing neutrophilic pulmonary inflammation by challenge to aerosolized lipopolysaccharide by nebulization, to model acute lung injury. In addition, basic surgical techniques for lung isolation, tracheal intubation and bronchoalveolar lavage are also described.

Explore More Videos

Pulmonary NeutrophiliaAerosolized LipopolysaccharideAcute Lung InjuryExperimental ModelPseudomonas AeruginosaBronchoalveolar LavageSterile Pulmonary Inflammation

Read Article

Cite this Article

Roos, A. B., Berg, T., Ahlgren, K. M., Grunewald, J., Nord, M. A Method for Generating Pulmonary Neutrophilia Using Aerosolized Lipopolysaccharide. J. Vis. Exp. (94), e51470, doi:10.3791/51470 (2014).

Download .ris file

Copy

Share Video

.

Copy