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A lungo termine intravitale immunofluorescenza Imaging di tessuto componenti della matrice con Epifluorescenza e due fotoni Microscopia
JoVE Journal
生物工学
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JoVE Journal 生物工学
Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy
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A lungo termine intravitale immunofluorescenza Imaging di tessuto componenti della matrice con Epifluorescenza e due fotoni Microscopia

18,744 Views

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09:00 min

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April 22, 2014

DOI:

10.3791/51388-v

DOI:
10.3791/51388-v

•

09:00 min
April 22, 2014

•

4 Views
, , , ,
1Institute of Bioengineering and Swiss Institute of Experimental Cancer Research (ISREC),École Polytechnique Fédérale de Lausanne, 2Department of Cell and Developmental Biology and Knight Cancer Institute,Oregon Health & Science University

概要

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La matrice extracellulare subisce sostanziale rimodellamento durante la guarigione delle ferite, l'infiammazione e tumorigenesi. Vi presentiamo un approccio immunofluorescenza intravitale romanzo per visualizzare le dinamiche di fibrillare come pure componenti della matrice maglia-come con alta risoluzione spaziale e temporale con epifluorescenza o microscopia a due fotoni.

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Cite this Article

Güç, E., Fankhauser, M., Lund, A. W., Swartz, M. A., Kilarski, W. W. Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy. J. Vis. Exp. (86), e51388, doi:10.3791/51388 (2014).

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