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单病毒原子力显微镜和超分辨率荧光成像的样品制备

JoVE Journal
生物学

This content is Free Access.

JoVE Journal 生物学

Sample Preparation for Single Virion Atomic Force Microscopy and Super-resolution Fluorescence Imaging

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单病毒原子力显微镜和超分辨率荧光成像的样品制备

9,300 Views

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05:31 min

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January 02, 2014

DOI:

10.3791/51366-v

DOI:

10.3791/51366-v

•

05:31 min

January 02, 2014

•

3 Views

Jeffery A Hodges^1,2, Saveez Saffarian^1,2

¹Department of Physics & Astronomy,University of Utah, ²Center for Cell and Genome,University of Utah

概要

通过超分辨率荧光成像或原子力显微镜（AFM）将病毒附着在表面上是单一病毒成像的要求。在这里，我们演示了一种样品制备方法，用于控制将病毒粘附到适合用于 AFM 和超分辨率荧光成像的玻璃表面。

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