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Sample Preparation for Single Virion Atomic Force Microscopy and Super-resolution Fluorescence Imaging
Bölümler
- 00:05Başlık
- 00:52Coverslip Cleaning in Preparation for Chemical Treatment
- 01:36Formation of the PLL-g-PEG Thin Film Layer
- 02:36Avidin Binding Enhancement
- 03:20Active Face Antibody Tether
- 04:24Results: Single Virion Imaging
- 05:06Conclusion
The attachment of virions to a surface is a requirement for single virion imaging by Super-resolution fluorescence imaging or atomic force microscopy (AFM). Here we demonstrate a sample preparation method for controlled adhesion of virions to glass surfaces suitable for use in AFM and super-resolution fluorescence imaging.
