Establishing the Minimal Bactericidal Concentration of an Antimicrobial Agent for Planktonic Cells (MBC-P) and Biofilm Cells (MBC-B)

Thien-Fah Mah

doi:10.3791/50854

/

Establishing the Minimal Bactericidal Concentration of an Antimicrobial Agent for Planktonic Cells (MBC-P) and Biofilm Cells (MBC-B)

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Establishing the Minimal Bactericidal Concentration of an Antimicrobial Agent for Planktonic Cells (MBC-P) and Biofilm Cells (MBC-B)

19,629 Views

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06:36 min

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January 02, 2014

DOI:

10.3791/50854-v

DOI:

10.3791/50854-v

•

06:36 min

January 02, 2014

•

9 Views

Thien-Fah Mah¹

¹Department of Biochemistry, Microbiology and Immunology,University of Ottawa

概要

This protocol allows for a direct comparison between planktonic and biofilm resistance for a bacterial strain that can form a biofilm in vitro using a 96-well microtiter plate. Planktonic or biofilm bacteria are exposed to serial dilutions of the antimicrobial agent of choice. Viability is assayed by growth on agar plates.

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Minimal Bactericidal Concentration MBC Antimicrobial Agent Planktonic Cells MBC-P Biofilm Cells MBC-B Bacterial Strain Microtiter Plate Antimicrobial Resistance Biofilm Formation Biofilm Recovery Cell Viability Assay

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Establishing the Minimal Bactericidal Concentration of an Antimicrobial Agent for Planktonic Cells (MBC-P) and Biofilm Cells (MBC-B)

概要

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