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Sex Stratified Neuronal Cultures to Study Ischemic Cell Death Pathways
JoVE Journal
神経科学
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JoVE Journal 神経科学
Sex Stratified Neuronal Cultures to Study Ischemic Cell Death Pathways

Sex Stratified Neuronal Cultures to Study Ischemic Cell Death Pathways

11,918 Views

10:44 min

December 09, 2013

DOI:

10:44 min
December 09, 2013

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概要

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Primary disassociated embryonic hippocampal neuronal cultures are useful for investigating the signaling mechanisms involved in neuron death. Sexing the embryos before the isolation and dissociation of the hippocampus allows the preparation of separate male and female cultures, which enables the researcher to identify and investigate sex-specific cell signaling.

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