Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa

Luca Signor; Elisabetta Boeri Erba

doi:10.3791/50635

/

Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa

JoVE Journal
化学

This content is Free Access.

JoVE Journal 化学

Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa

79,643 Views

•

07:49 min

•

September 09, 2013

DOI:

10.3791/50635-v

DOI:

10.3791/50635-v

•

07:49 min

September 09, 2013

•

25 Views

Luca Signor¹, Elisabetta Boeri Erba¹

¹Institute of Structural Biology “J.P. Ebel”, UMR5075, Commissariat à L’Energie Atomique et aux Energies Alternatives (CEA), Centre National de la Recherche Scientifique (CNRS),Université J. Fourier

概要

Accurate mass measurement represents an important step during the investigation of proteins. Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) can be used for such determination and its key advantage is the tolerance to salts, detergents and contaminants. Here we illustrate an accessible approach for the analysis of proteins larger than 100 kDa by MALDI-MS.

Explore More Videos

MALDI-TOF Mass Spectrometry Intact Proteins Protein Analysis Protein Characterization Large Proteins Protein Mass Determination Sample Preparation Matrix Calibration Laser Energy Acquisition Time

Read Article

Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa

概要

Automatically generated

Explore More Videos