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Üretim ve Farelerde arteriyel Lezyonlarının 3-Boyutlu kantitasyonu Optik Projeksiyon Tomografi ile
JoVE Journal
医学
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JoVE Journal 医学
Generation and 3-Dimensional Quantitation of Arterial Lesions in Mice Using Optical Projection Tomography
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Üretim ve Farelerde arteriyel Lezyonlarının 3-Boyutlu kantitasyonu Optik Projeksiyon Tomografi ile

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11:45 min

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May 26, 2015

DOI:

10.3791/50627-v

DOI:
10.3791/50627-v

•

11:45 min
May 26, 2015

•

2 Views
, , , , , , ,
1University/ BHF Centre for Cardiovascular Science, University of Edinburgh,The Queen’s Medical Research Institute

概要

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Ex vivo analysis of arterial lesions from animal models of cardiovascular disease classically relies on histological and immunohistochemical techniques. These provide 2-dimensional measurements in 3-dimensional lesions. This manuscript describes the generation of arterial lesions for quantitative analysis in 3-dimensions using optical projection tomography.

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Cite this Article

Kirkby, N. S., Low, L., Wu, J., Miller, E., Seckl, J. R., Walker, B. R., Webb, D. J., Hadoke, P. W. F. Generation and 3-Dimensional Quantitation of Arterial Lesions in Mice Using Optical Projection Tomography. J. Vis. Exp. (99), e50627, doi:10.3791/50627 (2015).

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