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Generation and 3-Dimensional Quantitation of Arterial Lesions in Mice Using Optical Projection Tomography
JoVE 杂志
医学
This content is Free Access.
JoVE 杂志 医学
Generation and 3-Dimensional Quantitation of Arterial Lesions in Mice Using Optical Projection Tomography

Generation and 3-Dimensional Quantitation of Arterial Lesions in Mice Using Optical Projection Tomography

DOI:
10.3791/50627-v

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11:45 min

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May 26, 2015

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, , , , , , ,
1University/ BHF Centre for Cardiovascular Science, University of Edinburgh,The Queen’s Medical Research Institute

Chapters

  • 00:05Title
  • 01:50Mouse Femoral Artery Lesion Surgery
  • 05:12Analyzing Arterial Lesion by Optical Projection Tomography (OPT)
  • 07:40Image Reconstruction and Analysis
  • 09:10Optical Projection Tomographs of Lesioned Femoral Arteries and Aortic Arch
  • 10:51Conclusion

Summary

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Ex vivo analysis of arterial lesions from animal models of cardiovascular disease classically relies on histological and immunohistochemical techniques. These provide 2-dimensional measurements in 3-dimensional lesions. This manuscript describes the generation of arterial lesions for quantitative analysis in 3-dimensions using optical projection tomography.

Tags

Optical Projection Tomography3D QuantitationArterial LesionsMiceAtherosclerosisNeointimal LesionsWire-insertionLigationApoE-deficientHistologyImmunohistochemistryVascular Remodeling

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