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Localizzazione e quantificazione relativa dei nanotubi di carbonio in cellule con immagini multispettrali Citometria a Flusso
JoVE Journal
生物工学
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JoVE Journal 生物工学
Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry
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Localizzazione e quantificazione relativa dei nanotubi di carbonio in cellule con immagini multispettrali Citometria a Flusso

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14:09 min

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December 12, 2013

DOI:

10.3791/50566-v

DOI:
10.3791/50566-v

•

14:09 min
December 12, 2013

•

1 Views
, , , , , ,
1Laboratoire Matière et Systèmes Complexes (MSC),CNRS/Université Paris Diderot, 2ImagoSeine BioImaging Core Facility, Institut Jacques Monod,CNRS/Université Paris Diderot, 3Laboratoire d’Immunopathologie et Chimie Thérapeutique,CNRS/Institut de Biologie Moléculaire et Cellulaire

概要

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In this study, the main purpose was to monitor the cellular uptake and eventual exocytosis of carbon nanotubes (CNTs). From this perspective, we proposed here a unique method using multispectral imaging flow cytometry allowing a quantification and localization of CNTs in a statistically relevant number of cells.

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Cite this Article

Marangon, I., Boggetto, N., Ménard-Moyon, C., Luciani, N., Wilhelm, C., Bianco, A., Gazeau, F. Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry. J. Vis. Exp. (82), e50566, doi:10.3791/50566 (2013).

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