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Tissue Engineering of a Human 3D in vitro Tumor Test System
JoVE Journal
生物工学
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JoVE Journal 生物工学
Tissue Engineering of a Human 3D in vitro Tumor Test System
DOI:

11:12 min

August 06, 2013

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  • 00:05標題
  • 02:16Decellularization of the BioVaSc
  • 03:52Isolation of Primary Human Dermal Microvascular Endothelial Cells (mvECs) and Fibroblasts
  • 05:50Tumor Test System A. Static Culture
  • 07:36Tumor Test System B. Dynamic Culture
  • 08:45Results: Immunohistological Characterization of 3D Tumor Models
  • 10:40Conclusion

概要

自動翻訳

Methods to create human 3D tumor tissues as test systems are described. These technologies are based on a decellularized Biological Vascularized Scaffold (BioVaSc), primary human cells and a tumor cell line, which can be cultured under static as well as under dynamic conditions in a flow bioreactor.

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