This content is Free Access.
Bir Zebra balığı Larva Lusıferaz Muhabir Sistemi ile glukokortikoid Signaling'den için bir Kimyasal Tarama Prosedürü
章
- 00:05標題
- 02:22Protocol
- 04:40Drug Treatment of Fish Larvae
- 07:09Luminescence Recording
- 07:52Data Analysis with Representative Results
- 12:01Conclusion
We describe the procedure and data analysis of a chemical screening system for glucocorticoid stress hormone signaling using zebrafish larvae: the Glucocorticoid Responsive In vivo Zebrafish Luciferase activitY (GRIZLY) assay. The assay sensitively and specifically detects effects on glucocorticoid signaling by compounds that require metabolization or affect endogenous glucocorticoid production.
