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Introduction to Solid Supported Membrane Based Electrophysiology
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JoVE Journal 生物学
Introduction to Solid Supported Membrane Based Electrophysiology

Introduction to Solid Supported Membrane Based Electrophysiology

DOI:
10.3791/50230-v

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19:56 min

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May 11, 2013

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, , , , ,
1Department of Biophysical Chemistry,Max Planck Institute of Biophysics, 2Buchmann Institute for Molecular Life Sciences,Goethe University Frankfurt

章

  • 00:01標題
  • 00:06Introduction
  • 01:25Background: Measuring principles
  • 02:51Background: The SSM Setup
  • 05:25Experimental procedure: Mounting the cuvette
  • 08:34Experimental procedure: Checking membrane parameters
  • 09:47Experimental procedure: Adding the protein sample
  • 10:53Experimental procedure: Defining the flow protocol
  • 11:48Experimental procedure: Measuring transporter signals
  • 14:12Data Analysis: Reconstruction of the transporter current
  • 15:07Data Analysis: Representative results
  • 17:26Data Analysis: Artifacts & control measurements
  • 19:00Conclusion

概要

自動翻訳

自動翻訳

Here we present an electrophysiological method based on solid supported membranes with focus on its applications for the characterization of electrogenic membrane transporters.

タグ

Solid Supported Membrane電気生理学Membrane ProteinProteoliposomesTransporterCapacitive CouplingTransient CurrentsSolution Exchange

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