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Isolatie en Cultuur van individuele myovezels en hun satelliet cellen van volwassen skeletspier
JoVE Journal
生物学
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JoVE Journal 生物学
Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle
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Isolatie en Cultuur van individuele myovezels en hun satelliet cellen van volwassen skeletspier

61,383 Views

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11:57 min

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March 22, 2013

DOI:

10.3791/50074-v

DOI:
10.3791/50074-v

•

11:57 min
March 22, 2013

•

42 Views
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1Sprott Center for Stem Cell Research,Ottawa Hospital Research Institute, 2Department of Cellular and Molecular Medicine,University of Ottawa

概要

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Isolatie en cultuur van myovezels is de gouden standaard<em> In vitro</em> Om de overgang van satelliet-cellen door middel van rust, activering en differentiatie te bestuderen. Belangrijk is dat de enige myofiber cultuur systeem bewaart de myofiber / stamcel vereniging, die is een essentieel onderdeel van de spier stamcel niche.

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Cite this Article

Pasut, A., Jones, A. E., Rudnicki, M. A. Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle. J. Vis. Exp. (73), e50074, doi:10.3791/50074 (2013).

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