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Imaging Analysis of Neuron to Glia Interaction in Microfluidic Culture Platform (MCP)-based Neuronal Axon and Glia Co-culture System
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October 14, 2012
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This study describes the procedures of setting up a novel neuronal axon and (astro)glia co-culture platform. In this co-culture system, manipulation of direct interaction between a single axon (and single glial cell) becomes feasible, allowing mechanistic analysis of the mutual neuron to glial signaling.
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Imaging AnalysisNeuronGlia InteractionMicrofluidic Culture PlatformMCP-based Neuronal Axon And Glia Co-culture SystemSignaling PathwaysCentral Nervous System (CNS)Neuron And Glia Mixed CulturesNeuronal CompartmentsAxonDendriteSomaCulture SystemSoluble FactorsDirect Membrane Contact SignalsSingle AxonGlial Cell
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Higashimori, H., Yang, Y. Imaging Analysis of Neuron to Glia Interaction in Microfluidic Culture Platform (MCP)-based Neuronal Axon and Glia Co-culture System. J. Vis. Exp. (68), e4448, doi:10.3791/4448 (2012).
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