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Ex vivo Culturing of Whole, Developing Drosophila Brains
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July 27, 2012
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概要
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This article describes a method by which one can mimic in vivo development of the Drosophila mushroom body in an ex vivo culture system.
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Ex Vivo CulturingDrosophila BrainsChronic Genetic ManipulationsCell BiologyDevelopmentCentral Brain StructuresPharmacological InterventionsLive ImagingSubcellular CompartmentAxon Initial Segment (AIS)Neuron-specific Cyclin-dependent KinaseCdk5Pharmacological InhibitorsActin OrganizationCell-surface Protein Fasciclin 2MutantsRemodelingConnectionsEmbryonic Mushroom Body (MB)MetamorphosisLarva To AdultOlfactory Learning And Memory
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Cite this Article
Prithviraj, R., Trunova, S., Giniger, E. Ex vivo Culturing of Whole, Developing Drosophila Brains. J. Vis. Exp. (65), e4270, doi:10.3791/4270 (2012).
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