このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry
23,383 Views
•
12:31 min
•
August 19, 2012
DOI:
概要
Automatically generated
Adenovirus particles are engineered to contain either the unnatural amino acid analogue azidohomoalanine or the azido sugar O-GlcNAz. The azide group of each is chemoselectively ligated via "click" chemistry reactions as a means of viral surface modification.
Explore More Videos
Chemoselective ModificationViral SurfacesBioorthogonal Click ChemistryGene TherapyOncolytic ApplicationsVaccine DevelopmentGenetics-based ApproachesChemoselective LigationTargeting MoleculesDyesAzide TagsMetabolic LabelingEffector LigandsAdenovirus ParticlesAzidohomoalanineO-linked N-azidoacetylglucosamine
Read Article
Cite this Article
Rubino, F. A., Oum, Y. H., Rajaram, L., Chu, Y., Carrico, I. S. Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry. J. Vis. Exp. (66), e4246, doi:10.3791/4246 (2012).
Copy