ジャーナル
/
/
A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
JoVE Journal
免疫学と感染
This content is Free Access.
JoVE Journal 免疫学と感染
A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

17,537 Views

13:30 min

November 07, 2012

DOI:

13:30 min
November 07, 2012

1 Views
,

概要

Automatically generated

A novel directed evolution method specific to the field of thermostability engineering was developed and consequently validated for bacteriolytic enzymes. After only one round of random mutagenesis, an evolved bacteriolytic enzyme, PlyC 29C3, displayed greater than twice the residual activity when compared to the wild-type protein after elevated temperature incubation.

Read Article