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Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting
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生物学
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JoVE Journal 生物学
Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting

Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting

DOI:
10.3791/4214-v

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11:25 min

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October 04, 2012

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, , , ,
1School of Life Sciences,University of Warwick , 2Warwick Systems Biology,University of Warwick

章

  • 00:05標題
  • 02:10Protoplast Generation
  • 04:23FACS of Leaf Protoplasts
  • 05:54Results: Representative Analyses of GFP Expression in Flow Sorted Arabidopsis Protoplasts
  • 10:26Conclusion

概要

自動翻訳

自動翻訳

A method for producing Arabidopsis leaf protoplasts that are compatible with fluorescence activated cell sorting (FACS), allowing for studies of specific cell populations. This method is compatible with any Arabidopsis line that expresses GFP in a subset of cells.

タグ

Arabidopsis LeavesFluorescence Activated Cell SortingBiomass AccumulationCell ProliferationCell ExpansionLeaf PrimordiumLeaf OrganCell TypesLeaf Development StagesSenescenceGenomic StudyExpression DataGenetic NetworksCellular Response ZonesCell Specific Gene ExpressionLaser-capture Microdissection (LCM)GFP Expressing PlantsProtoplast GenerationINTACT SystemNuclear PrecipitationImmunoprecipitation Of Polysomes

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