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LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations
JoVE Journal
化学
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JoVE Journal 化学
LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations

LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations

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09:32 min

February 04, 2013

DOI:

09:32 min
February 04, 2013

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概要

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Ice binding proteins (IBPs), also known as antifreeze proteins, inhibit ice growth and are a promising additive for use in the cryopreservation of tissues. The main tool used to investigate IBPs is the nanoliter osmometer. We developed a home-designed cooling stage mounted on an optical microscope and controlled using a custom-built LabVIEW routine. The nanoliter osmometer described here manipulated the sample temperature in an ultra-sensitive manner.

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