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Conversion of a Capture ELISA to a Luminex xMAP Assay using a Multiplex Antibody Screening Method
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July 06, 2012
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An ELISA can be easily converted to a Luminex xMAP assay and, through the benefits of multiplexing, several antibodies can be screened simultaneously to identify an optimum antibody pair, resulting in increased sensitivity and dynamic range, while reducing assay cost.
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ELISALuminex XMAP AssayAntibody ScreeningMultiplexCapture AntibodyAnalytesBiological SamplesLife Science ResearchClinical DiagnosticsLimitations96-well MicroplateSample VolumeAntigenNon-specific BindingBackgroundEnzyme-mediated AmplificationSensitivityTechnologyHigher ThroughputFlexibilityReduced CostWorkflowLuminex XMAP TechnologyMicrosphere Array PlatformMonoplex AssayProtein ApplicationsNucleic Acid Applications
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Baker, H. N., Murphy, R., Lopez, E., Garcia, C. Conversion of a Capture ELISA to a Luminex xMAP Assay using a Multiplex Antibody Screening Method. J. Vis. Exp. (65), e4084, doi:10.3791/4084 (2012).
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