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Odorant-induced Responses Recorded from Olfactory Receptor Neurons using the Suction Pipette Technique
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April 05, 2012
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Olfactory receptor neurons (ORNs) convert odor signals first into a receptor current that in turn triggers action potentials that are conveyed to second order neurons in the olfactory bulb. Here we describe the suction pipette technique to record simultaneously the odorant-induced receptor current and action potentials from mouse ORNs.
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Odorant-induced ResponsesOlfactory Receptor NeuronsSuction Pipette TechniqueChemosensory SystemsNasal CavityOlfactory EpitheliumSignal Transduction MachineryExcitatory Current InfluxCiliary Transduction ChannelsCyclic Nucleotide-gated ChannelCa2+-activated Cl- ChannelDepolarizationAction Potential GenerationSuction Pipette Technique For Recording ORN Responses
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Ponissery Saidu, S., Dibattista, M., Matthews, H. R., Reisert, J. Odorant-induced Responses Recorded from Olfactory Receptor Neurons using the Suction Pipette Technique. J. Vis. Exp. (62), e3862, doi:10.3791/3862 (2012).
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