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The Neuroblast Assay: An Assay for the Generation and Enrichment of Neuronal Progenitor Cells from Differentiating Neural Stem Cell Progeny Using Flow Cytometry
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JoVE Journal 神経科学
The Neuroblast Assay: An Assay for the Generation and Enrichment of Neuronal Progenitor Cells from Differentiating Neural Stem Cell Progeny Using Flow Cytometry

The Neuroblast Assay: An Assay for the Generation and Enrichment of Neuronal Progenitor Cells from Differentiating Neural Stem Cell Progeny Using Flow Cytometry

DOI:
10.3791/3712-v

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15:40 min

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April 22, 2012

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, , ,
1Department of Neurosurgery,The University of Florida, 2Laboratory for Stem Cell Research, Department of Anatomical Sciences,Shiraz University of Medical Sciences, Shiraz, Iran

章

  • 00:02標題
  • 01:46Starting Neuroblast Assay Culture
  • 04:07Switching Neuroblast Assay Medium
  • 04:55Cell Preparation for Flow Cytometry
  • 06:42Staining for PSA-NCAM
  • 08:31Isolation of Neuronal Progenitor Cells Using Flow Cytometry
  • 11:34Plating Sorted Cells
  • 13:12Representative Results
  • 14:34Conclusion

概要

自動翻訳

自動翻訳

This video protocol demonstrates a novel method for the generation and subsequent purification of neuronal progenitor cells from a renewable source of neural stem cells (NSCs) based on their physical (size and internal granularity) and fluorescent properties using flow cytometry technology.

タグ

Neuroblast AssayGeneration Of Neuronal Progenitor CellsDifferentiating Neural Stem CellsFlow CytometryNeurosphere AssayCentral Nervous SystemAstrocytesOligodendrocytesNeuronsIn Vitro StudiesDrug ScreeningNeurotoxicology電気生理学Cell Replacement TherapyHeterogeneity Of NSC ProgenyLow Production Of Neurons And OligodendrocytesPredominance Of AstrocytesClinical ApplicationsPurification Of Immature NeuronsFluorescence Activated Cell Sorting (FACS)Murine NSC ProgenyE14 Mouse Ganglionic Eminences

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