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Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
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JoVE Journal 免疫学と感染
Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance

Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance

DOI:
10.3791/3686-v

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10:41 min

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January 03, 2012

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, , ,
1Department of Molecular Biology and Microbiology,University of Central Florida

章

  • 00:05標題
  • 01:31Cell Intoxication and Permeabilization
  • 03:34Sample and Standard Toxin Preparation
  • 06:11Surface Plasmon Resonance
  • 07:08Representative Surface Plasmon Resonance Traces and Analysis
  • 10:18Conclusion

概要

自動翻訳

自動翻訳

In this report, we describe how surface plasmon resonance is used to detect toxin entry into the host cytosol. This highly sensitive method can provide quantitative data on the amount of cytosolic toxin, and it can be applied to a range of toxins.

タグ

Toxin TranslocationSurface Plasmon ResonanceAB ToxinsCell-binding B SubunitVesicle CarriersEndoplasmic Reticulum (ER)Catalytic A ChainProtein-conducting ChannelCytosolic TargetToxin TraffickingLysosomesGolgi ApparatusSubcellular Fractionation ProtocolDigitonin PermeabilizationAnti-toxin A Chain AntibodyDetection Method

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