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鉴定蛋白质相互作用的合作伙伴使用串联亲和纯化
JoVE Journal
生物学
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JoVE Journal 生物学
Identification of Protein Interacting Partners Using Tandem Affinity Purification
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鉴定蛋白质相互作用的合作伙伴使用串联亲和纯化

37,062 Views

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10:02 min

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February 25, 2012

DOI:

10.3791/3643-v

DOI:
10.3791/3643-v

•

10:02 min
February 25, 2012

•

15 Views
, , ,
1Section of Virology, Department of Medicine,Imperial College London

概要

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串联亲和纯化的蛋白结合合作伙伴的标识是稳健的做法。由于概念证明，这种方法的应用以及特色的翻译起始因子eIF4E共沉淀的宿主细胞因子参与翻译起始。这种方法是很容易适应任何细胞或病毒蛋白。

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Cite this Article

Bailey, D., Urena, L., Thorne, L., Goodfellow, I. Identification of Protein Interacting Partners Using Tandem Affinity Purification. J. Vis. Exp. (60), e3643, doi:10.3791/3643 (2012).

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