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In vivo Electroporation of Morpholinos into the Regenerating Adult Zebrafish Tail Fin
章
- 00:05標題
- 01:19Morpholino Resuspension and Fin Amputation
- 02:37Morpholino Injection
- 04:05Electroporation
- 05:14Analysis
- 06:17Representative Fin-outgrowth Results
- 06:55Conclusion
We describe a method to conditionally knockdown the expression of a target protein during adult zebrafish fin regeneration. This technique involves micro-injecting and electroporating antisense oligonucleotide morpholinos into fin tissue, which allows testing the protein’s role in various stages of fin regeneration, including wound healing, blastema formation, and regenerative outgrowth.
タグ
In Vivo ElectroporationMorpholinosRegenerating Adult Zebrafish Tail FinUrodelesTeleost FishZebrafish ModelSpontaneous RegenerationHeart RegenerationRetina RegenerationSpinal Cord RegenerationOptic Nerve RegenerationSensory Hair Cell RegenerationFin Regeneration StagesWound HealingBlastema FormationFin OutgrowthEpithelium ProliferationCell Cycle Re-entryProliferative Blastema FormationRe-differentiation Of CellsGene Expression During Fin Regeneration
