ジャーナル
/
生物学
/
由一个小型的双特异性亲和标签的推动正交蛋白质纯化
JoVE Journal
生物学
This content is Free Access.
JoVE Journal 生物学
Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag
Please note that all translations are automatically generated. Click here for the English version.

由一个小型的双特异性亲和标签的推动正交蛋白质纯化

16,839 Views

•

10:32 min

•

January 16, 2012

DOI:

10.3791/3370-v

DOI:
10.3791/3370-v

•

10:32 min
January 16, 2012

•

1 Views
, ,
1School of Biotechnology, Department of Proteomics,Royal Institute of Technology

概要

Automatically generated

已开发一种新的和高效两步亲和层析协议，并进行了详细描述。该方法是基于一个小净化标签有两个固有的亲和力，适用于广泛的目标蛋白质具有不同的属性。

Explore More Videos

Orthogonal Protein PurificationSmall Bispecific Affinity TagRecombinant ProteinsPurification ProtocolsHigh-throughput MethodsPurification TagsHexa-histidine TagAffinity ChromatographyBispecific Purification TagAlbumin-binding DomainStreptococcal Protein GHuman Serum Albumin (HSA)Phage Display TechnologyDimeric Z-domainStaphylococcal Protein A

Read Article

Cite this Article

Nilvebrant, J., Alm, T., Hober, S. Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag. J. Vis. Exp. (59), e3370, doi:10.3791/3370 (2012).

Download .ris file

Copy

Share Video

.

Copy