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Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag
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January 16, 2012
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A novel and highly efficient two-step affinity chromatography protocol has been developed and is described in detail. The method is based on a small purification tag with two inherent affinities and is applicable to a wide range of target proteins with different properties.
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Nilvebrant, J., Alm, T., Hober, S. Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag. J. Vis. Exp. (59), e3370, doi:10.3791/3370 (2012).
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