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High-throughput Saccharification Assay for Lignocellulosic Materials
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July 03, 2011
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概要
Automatically generated
A simple, rapid method for determining the saccharification potential of large numbers of plant biomass samples is described. The automated platform for this analysis involves the preparation of the plant biomass for analysis in 96 well plates and the subsequent performance of pretreatment, hydrolysis and quantification of the sugars released.
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High-throughput Saccharification AssayLignocellulosic MaterialsPolysaccharidesPlant BiomassBiorefinerySustainableCarbon NeutralFossil Fuel DependencyRecalcitrancePlant Cell WallsCrystalline CelluloseMatrix PolysaccharidesXylansArabinoxylansPhenolic Polymer LigninDigestibilityBottlenecksSaccharification Of Cell WallsMutant PopulationsBreeding PopulationsVariability In SaccharificationHigh Throughput ApproachAnalytical Platform96-well Plate FormatScreeningLarge PopulationsPlant Species
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Gomez, L. D., Whitehead, C., Roberts, P., McQueen-Mason, S. J. High-throughput Saccharification Assay for Lignocellulosic Materials. J. Vis. Exp. (53), e3240, doi:10.3791/3240 (2011).
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