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Cercarial Transformation and in vitro Cultivation of Schistosoma mansoni Schistosomules
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August 16, 2011
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We describe an in vitro method for culturing schistosomula of the flatworm parasite Schistosoma mansoni, via the harvesting and transformation of infective cercariae from the fresh water snail intermediate host Biomphalaria glabrata.
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Cercarial TransformationIn Vitro CultivationSchistosoma MansoniSchistosomulesSchistosomiasisParasitic DiseaseTrematode WormsLife CycleMolluscan HostMammalian HostFree-swimming StagesCercariaeSecreted ProteasesSchistosomulesHost Immune SystemRed Blood Cells DigestionLungsPortal CirculationHepatic Portal SystemMesenteric VeinsMale And Female Worms Pairing And MatingEgg ProductionMiracidiaSnail SpeciesSporocysts
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Milligan, J. N., Jolly, E. R. Cercarial Transformation and in vitro Cultivation of Schistosoma mansoni Schistosomules. J. Vis. Exp. (54), e3191, doi:10.3791/3191 (2011).
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