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Klonal Genişleme CD133 + Karaciğer Kök Hücre İzolasyonu
JoVE Journal
生物学
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JoVE Journal 生物学
Isolation of CD133+ Liver Stem Cells for Clonal Expansion
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Klonal Genişleme CD133 + Karaciğer Kök Hücre İzolasyonu

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12:06 min

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October 10, 2011

DOI:

10.3791/3183-v

DOI:
10.3791/3183-v

•

12:06 min
October 10, 2011

•

4 Views
, , , ,
1Department of Pediatrics and Pharmacology,Pennsylvania State College of Medicine, 2Department of Pharmacology,Pennsylvania State College of Medicine, 3小児科,University of California Los Angeles, School of Medicine

概要

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İşte biz bütün fare karaciğer doku sindirim, hücre zenginleştirme ve flow sitometri izolasyonu gerektiren bir süreçten CD133 eksprese karaciğer kök hücre ve kanser kök hücrelerin izolasyonu tarif. Biz gelişmiş tek hücre izolasyonu ve klonal genişleme için yöntemler içerir.

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Cite this Article

Rountree, C. B., Ding, W., Dang, H., VanKirk, C., Crooks, G. M. Isolation of CD133+ Liver Stem Cells for Clonal Expansion. J. Vis. Exp. (56), e3183, doi:10.3791/3183 (2011).

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