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Drosophila Pupal Abdomen Immunohistochemistry
JoVE Journal
生物学
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JoVE Journal 生物学
Drosophila Pupal Abdomen Immunohistochemistry

Drosophila Pupal Abdomen Immunohistochemistry

DOI:
10.3791/3139-v

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08:29 min

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October 02, 2011

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,
1Department of Biological Sciences,University of Alabama

章

  • 00:05標題
  • 01:19Collection, Culturing and Staging Pupae
  • 02:33Dissection
  • 03:40Cleaning, Fixation and Primary Antibody Incubation
  • 05:38Secondary Antibody Incubation and Mounting
  • 07:24Images of Prepared Samples
  • 08:00Conclusion

概要

自動翻訳

自動翻訳

Antibody staining of the Drosophila pupae can enhance genetic analyses of adult abdominal developmental genetics. We present our protocol for dissection, fixation and antibody staining of staged Drosophila pupal abdomen.

タグ

DrosophilaPupal AbdomenImmunohistochemistryEpithelial MorphogenesisSexually Dimorphic MorphologiesImaginal CellsHistoblast NestsLarval EpidermisAdult Abdominal SegmentsDorsal CuticleVentral CuticleSpiraclesDiploid CellsLarval Epidermal CellsMolecular ToolsGenetic PathwaysAdult PhenotypesDissectionImmunohistochemical Analyses

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