Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Aaron A. Rowe; Ryan J. White; Andrew J. Bonham; Kevin W. Plaxco

doi:10.3791/2922

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Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

JoVE Journal
生物工学

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JoVE Journal 生物工学

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

32,683 Views

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13:15 min

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June 01, 2011

DOI:

10.3791/2922-v

DOI:

10.3791/2922-v

•

13:15 min

June 01, 2011

•

27 Views

Aaron A. Rowe¹, Ryan J. White¹, Andrew J. Bonham¹, Kevin W. Plaxco²

¹Department of Chemistry and Biochemistry,University Of California Santa Barbara, ²Department of Chemistry and Biochemistry, Program in BioMolecular Science and Engineering,University Of California Santa Barbara

概要

"E-DNA" sensors, reagentless, electrochemical biosensors that perform well even when challenged directly in blood and other complex matrices, have been adapted to the detection of a wide range of nucleic acid, protein and small molecule analytes. Here we present a general procedure for the fabrication and use of such sensors.

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Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

概要

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