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Flow Cytometry Analysis of Immune Cells Within Murine Aortas
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JoVE Journal 免疫学と感染
Flow Cytometry Analysis of Immune Cells Within Murine Aortas

Flow Cytometry Analysis of Immune Cells Within Murine Aortas

34,098 Views

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15:15 min

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July 01, 2011

DOI:

10.3791/2848-v

DOI:
10.3791/2848-v

•

15:15 min
July 01, 2011

•

25 Views
, , ,
1Deptartment of Microbiology and Molecular Cell Biology,Eastern Virginia Medical School, 2Division of Inflammation Biology,LaJolla Institute for Allergy and Immunology

概要

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This paper presents a flow cytometry-based method to investigate the immune composition of aortas. The paper also illustrates an additional technique that allows examining surrounding adventitia and vessel wall separately. This method opens possibilities to perform phenotypical analyses of aortic leukocytes and apply several immunological assays for atherosclerosis studies.

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Butcher, M. J., Herre, M., Ley, K., Galkina, E. Flow Cytometry Analysis of Immune Cells Within Murine Aortas. J. Vis. Exp. (53), e2848, doi:10.3791/2848 (2011).

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