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Kemirgen Beyin Dilimleri Nöronlar Vibrodissociation Sinaptik İletim ve Görüntü Presinaptik Terminalleri Eğitim
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08:38 min
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May 25, 2011
DOI:
1Section on Synaptic Pharmacology/Laboratory for Integrative Neuroscience,National Institutes of Health/National Institute on Alcohol Abuse and Alcoholism, 2Department of Electronics Engineering,Ewha Womans University, 3Section on Transmitter Signaling/Laboratory of Molecular Physiology,National Institutes of Health/National Institute on Alcohol Abuse and Alcoholism
概要
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Bu rapor gösteriyor bağlı presinaptik BOUTONS istinat bireysel canlı nöronların mekanik izolasyon için kullanılan bir tekniktir. Vibrodissociated nöronlar, hızlı üretim, mükemmel farmakolojik kontrolü ve komşu hücrelerden etkisi olmadan gelişmiş uzay-klemp avantajlara sahip. Bu yöntem, sinaptik elemanları ve yama klemp kayıt görüntüleme için kullanılabilir.
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Cite this Article
Jun, S. B., Cuzon Carlson, V., Ikeda, S., Lovinger, D. Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals. J. Vis. Exp. (51), e2752, doi:10.3791/2752 (2011).
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