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Isolation of Drosophila melanogaster Testes
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May 13, 2011
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概要
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Drosophila melanogaster testes can be rapidly and efficiently isolated from adult males using dissecting needles. With practice, one can readily isolate in one or two days an amount of testes sufficient for the analysis of DNA or RNA by high throughput sequencing or more traditional molecular biology methods or of protein for antibody- or enzyme-based assays.
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Drosophila Melanogaster TestesStem Cell MaintenanceDifferentiationMeiosisSoma-germline InteractionsTestes IsolationAdult MalesEclosionPupal CaseWild-type FliesWhite Mutant FliesGenetic BackgroundLaboratory ExperimentsGlass Microscope SlideBlack BackgroundIdentifying TestesDissecting NeedlesForcepsQuicker Method200-300 Wild-type Flies Per Hour400-600 Testes YieldTestes Size Reduction200-400 Testes Per Hour
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Zamore, P. D., Ma, S. Isolation of Drosophila melanogaster Testes. J. Vis. Exp. (51), e2641, doi:10.3791/2641 (2011).
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