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Organotypic Cerebellar Cultures: Apoptotic Challenges and Detection
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神経科学
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JoVE Journal 神経科学
Organotypic Cerebellar Cultures: Apoptotic Challenges and Detection

Organotypic Cerebellar Cultures: Apoptotic Challenges and Detection

DOI:
10.3791/2564-v

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11:57 min

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May 17, 2011

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, , ,
1Laboratory of Genetics,The Salk Institute for Biological Studies, 2Clayton Foundation Laboratories for Peptide Biology,The Salk Institute for Biological Studies

章

  • 00:05標題
  • 02:09Preparation of Organotypic Cerebellar Cultures
  • 05:53Fas Treatment and Staining of Cerebellar Slices
  • 09:49Examples of Cerebellar Slice Cultures
  • 10:49Conclusion

概要

自動翻訳

自動翻訳

This method describes the generation of organotypic cerebellar cultures and the effect of certain apoptotic stimuli on the viability of different cerebellar cell types.

タグ

Organotypic Cerebellar CulturesApoptotic ChallengesDetection神経科学Organotypic CulturesPreparationSlicesStaining ProcedureBrain PartsHippocampusCerebellumCellular OrganizationNeuronal NetworksPurkinje CellsDissociated Primary CultureDevelopmental FeaturesIn Vitro StudiesElectrophysiological ExperimentsPharmacological ExperimentsApoptotic StimuliFas LigandTUNEL StainingCell Death Measurement

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