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Efficient Polyethylene Glycol (PEG) Mediated Transformation of the Moss Physcomitrella patens
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生物学
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JoVE Journal 生物学
Efficient Polyethylene Glycol (PEG) Mediated Transformation of the Moss Physcomitrella patens

Efficient Polyethylene Glycol (PEG) Mediated Transformation of the Moss Physcomitrella patens

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04:54 min

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April 19, 2011

DOI:

10.3791/2560-v

DOI:
10.3791/2560-v

•

04:54 min
April 19, 2011

•

37 Views
,
1Department of Biology and Biotechnology,Worcester Polytechnic Institute- WPI

概要

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A simple and efficient method to transform Physcomitrella pantens protoplasts is described. This method is adapted from protocols for Physocmitrella protonemal protoplast and Arabidopsis mesophyll protoplast transformation1.

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Cite this Article

Liu, Y., Vidali, L. Efficient Polyethylene Glycol (PEG) Mediated Transformation of the Moss Physcomitrella patens. J. Vis. Exp. (50), e2560, doi:10.3791/2560 (2011).

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