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Scheiden Kralen en cellen in Multi-channel Microfluïdische apparaten met behulp van Dielectrophoresis en Laminar Flow
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生物工学
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JoVE Journal 生物工学
Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
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Scheiden Kralen en cellen in Multi-channel Microfluïdische apparaten met behulp van Dielectrophoresis en Laminar Flow

27,187 Views

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09:45 min

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February 04, 2011

DOI:

10.3791/2545-v

DOI:
10.3791/2545-v

•

09:45 min
February 04, 2011

•

3 Views
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1Electrical and Computer Engineering,University of Illinois at Urbana-Champaign, 2Micro and Nanotechnology Lab,University of Illinois at Urbana-Champaign, 3Mechanical Science and Engineering,University of Illinois at Urbana-Champaign, 4生物工学,University of Illinois at Urbana-Champaign

概要

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Dielectrophoresis (DEP) is een effectieve methode om cellen te manipuleren. Printed circuit boards (PCB's) kan bieden goedkope, herbruikbare en effectief elektroden voor contact-free celmanipulatie binnen microfluïdische apparaten. Door het combineren van PDMS-based microfluïdische kanalen met dekglaasjes op PCB's, tonen we aan kralen en cel-manipulatie en scheiding binnen multichannel microfluïdische apparaten.

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Microfluidic DevicesCell StudiesFluidic EnvironmentManipulating CellsSorting CellsCounting CellsDielectrophoresisLaminar FlowPrinted Circuit Boards (PCBs)Non-contact ManipulationBead ManipulationCell ManipulationBioactuationCell SeparationMultichannel Microfluidic DevicesDEP Electrodes

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Cite this Article

Millet, L. J., Park, K., Watkins, N. N., Hsia, K. J., Bashir, R. Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow. J. Vis. Exp. (48), e2545, doi:10.3791/2545 (2011).

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