This content is Free Access.
Detection of Functional Matrix Metalloproteinases by Zymography
82,811 Views
•
09:30 min
•
November 08, 2010
DOI:
概要
Automatically generated
This protocol describes an activity-based assay for detecting matrix metalloproteinases in culture supernatants or body fluids.
Explore More Videos
Functional Matrix MetalloproteinasesZymographyMMPsZinc-containing EndopeptidasesPeptide BondsCollagenasesGelatinasesMT-MMPStromelysinsMatrilysinsCell InvasionCartilage DegradationWound Healing胚発生Rheumatoid ArthritisCancerChronic Obstructive Pulmonary DiseaseMMP-2Gelatinase AType IV CollagenaseCell Culture SupernatantsSemi-quantitative TechniquePolyacrylamide GelSodium Dodecyl Sulfate (SDS)Gelatin Substrate
Read Article
Cite this Article
Hu, X., Beeton, C. Detection of Functional Matrix Metalloproteinases by Zymography. J. Vis. Exp. (45), e2445, doi:10.3791/2445 (2010).
Copy