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Detection of Functional Matrix Metalloproteinases by Zymography
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JoVE Journal Biologie
Detection of Functional Matrix Metalloproteinases by Zymography

Detection of Functional Matrix Metalloproteinases by Zymography

82,593 Views

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09:30 min

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November 08, 2010

DOI:

10.3791/2445-v

DOI:
10.3791/2445-v

•

09:30 min
November 08, 2010

•

8 Views
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1Department of Molecular Physiology and Biophysics,Baylor College of Medicine

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Functional Matrix MetalloproteinasesZymographyMMPsZinc-containing EndopeptidasesPeptide BondsCollagenasesGelatinasesMT-MMPStromelysinsMatrilysinsCell InvasionCartilage DegradationWound HealingEmbryogenesisRheumatoid ArthritisCancerChronic Obstructive Pulmonary DiseaseMMP-2Gelatinase AType IV CollagenaseCell Culture SupernatantsSemi-quantitative TechniquePolyacrylamide GelSodium Dodecyl Sulfate (SDS)Gelatin Substrate

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Hu, X., Beeton, C. Detection of Functional Matrix Metalloproteinases by Zymography. J. Vis. Exp. (45), e2445, doi:10.3791/2445 (2010).

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