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切片的制备，组织器官型培养，并记录在视交叉上核时钟基因活性的荧光素酶
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February 15, 2011
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概要
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准备含有成年小鼠的下丘脑视交叉上核（SCN），和快速的方式来培养器官培养条件的SCN组织切片，程序报告。此外，使用动态的荧光素酶报告技术的振荡时钟基因蛋白表达的测量是描述。
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Savelyev, S. A., Larsson, K. C., Johansson, A., Lundkvist, G. B. S. Slice Preparation, Organotypic Tissue Culturing and Luciferase Recording of Clock Gene Activity in the Suprachiasmatic Nucleus. J. Vis. Exp. (48), e2439, doi:10.3791/2439 (2011).
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