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Slice Preparation, Organotypic Tissue Culturing and Luciferase Recording of Clock Gene Activity in the Suprachiasmatic Nucleus
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Slice Preparation, Organotypic Tissue Culturing and Luciferase Recording of Clock Gene Activity in the Suprachiasmatic Nucleus
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February 15, 2011
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The procedure of preparing slices containing the adult mouse hypothalamic suprachiasmatic nucleus (SCN), and a rapid way to culture the SCN tissue in organotypic culture condition, are reported. Further, the measurement of oscillatory clock gene protein expression using dynamic luciferase reporter technology is described.
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Slice PreparationLuciferase RecordingClock Gene ActivitySuprachiasmatic NucleusCircadian ClockPhysiologyComportamientoRetinaOptic NerveNegative Feedback LoopsClock GenesPeriod GenesCore ClockMembrane DepolarizationCalciumCAMPOscillationsGene ExpressionMetabolic ActivityElectrical ActivityIn Vitro StudyMolecular InvestigationsElectrophysiological InvestigationsMetabolic Investigations
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Savelyev, S. A., Larsson, K. C., Johansson, A., Lundkvist, G. B. S. Slice Preparation, Organotypic Tissue Culturing and Luciferase Recording of Clock Gene Activity in the Suprachiasmatic Nucleus. J. Vis. Exp. (48), e2439, doi:10.3791/2439 (2011).
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