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在开发和成年阶段的中介代谢通量的稳定同位素分析线虫</em
JoVE Journal
生物学
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JoVE Journal 生物学
Stable Isotopic Profiling of Intermediary Metabolic Flux in Developing and Adult Stage Caenorhabditis elegans
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在开发和成年阶段的中介代谢通量的稳定同位素分析线虫</em

DOI:
10.3791/2288-v

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12:10 min

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February 27, 2011

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, , , , ,
1小児科,The Children’s Hospital of Philadelphia, 2小児科,University of Pennsylvania

章

  • 00:05標題
  • 01:51Preparing Worm Samples for Metabolite Quantitation
  • 04:37Processing Neutralized Samples for Amino Acid and Organic Acid Analysis by GC/MS
  • 07:11Monitoring Isotopic Utilization in Worms on Plates by Tracing Labeled Carbon in Atmospheric Carbon Dioxide
  • 08:39Results of Stable Isotopic Profiling of Intermediary Metabolism and Metabolic Flux
  • 11:40Conclusion

概要

自動翻訳

自動翻訳

稳定同位素分析气相色谱质谱中介代谢通量的质谱分析中所述的线虫，<em>线虫</em>。详细的评估中的二氧化碳，有机酸，氨基酸和同位素暴露在琼脂平板上的发展，或在液体培养成年的同位素富集的方法。

タグ

Stable Isotopic ProfilingIntermediary MetabolismMetabolic FluxCaenorhabditis ElegansNematode Growth Media Agar PlatesPharmacologic TreatmentsHigh Performance Liquid Chromatography (HPLC)Mass SpectrometryCarbon DioxideGlycolysisPyruvate MetabolismTricarboxylic Acid CycleIsotope Exposure TimeBacterial Clearing ProtocolsWorm Disruption Methods

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