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İnsan iPS hücreleri dondurularak ve kurtarma Tam nakavt Serum Yedek Besleyici-Alerjik Orta
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July 15, 2010
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概要
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Bu protokol nakavt SR kriyoprezervasyon orta insan iPS hücreleri dondurularak ve bu hücrelerin tam nakavt SR Besleyici Ücretsiz (KSR-FF), orta ya da besleyici tabanlı nakavt SR orta kurtarmak için ayrıntılı prosedürü anlatılmaktadır.
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CryopreservingRecoveringHuman IPS CellsComplete KnockOut Serum ReplacementFeeder-free MediumReprogrammingFibroblastsPluripotent CellsDrug DiscoveryCell TherapyBasic ResearchGIBCO Media And ReagentsKnockout DMEMKnockout Serum ReplacementEmbryonic Stem Cell GrowthIPS Cell CultureFeeder-free CultureKnockout SR Growth Factor CocktailTraditional Culture MethodsMouse Or Human Fibroblast Feeder LayersFeeder-conditioned MediumLabor-intensiveHard To ScaleUndifferentiated Cells
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Wagner, K., Welch, D. Cryopreserving and Recovering of Human iPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium. J. Vis. Exp. (41), e2237, doi:10.3791/2237 (2010).
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