Fluorescence imaging is a promising innovative modality for image-guided surgery in surgical oncology. In this video we describe the technical procedure for detection of the sentinel lymph node using fluorescence imaging as showcased in gynecologic oncologicy. A multispectral fluorescence camera system, together with the fluorescent agent indocyanine green, is applied.
Crane, L. M., Themelis, G., Buddingh, K. T., Harlaar, N. J., Pleijhuis, R. G., Sarantopoulos, A., van der Zee, A. G., Ntziachristos, V., van Dam, G. M. Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology. J. Vis. Exp. (44), e2225, doi:10.3791/2225 (2010).