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Visualization of Mitochondrial DNA Replication in Individual Cells by EdU Signal Amplification
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November 15, 2010
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We developed a sensitive technique to label newly synthesized mitochondrial DNA (mtDNA) in individual cells in order to study mtDNA biogenesis. The technique combines the incorporation of EdU together with a tyramide signal amplification (TSA) protocol to visualize mtDNA replication within subcellular compartments of neurons.
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Haines, K. M., Feldman, E. L., Lentz, S. I. Visualization of Mitochondrial DNA Replication in Individual Cells by EdU Signal Amplification. J. Vis. Exp. (45), e2147, doi:10.3791/2147 (2010).
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