siRNA Encapsulation into Exosomes Using Electroporation: An Electric Pulse Based Technique to Load siRNA into Exosomes
筆記録
Before starting the electroporation, pre-chill the electroporation cuvette on ice for 30 minutes. Mix 7 micrograms of exosomes with 0.33 micrograms of siRNA in the microcentrifuge tube. Add a citric acid buffer to achieve the volume of 150 microliters. Add the exosome-siRNA mixture to the electroporation cuvette using a plastic pipette and cap the cuvette.
Place the cuvette in the right orientation in the cuvette holder of the electroporator and rotate the turning wheel of the electroporator 180 degrees clockwise. Select the desired program and press the Start button to start electroporation. The display will indicate a successful pulse.
Then, turn back the wheel 180 degrees counterclockwise and remove the cuvette. Use a plastic pipette to remove the sample from the cuvette into a new microcentrifuge tube. Keep the tube on ice or in a fridge before further processing if not used immediately.
