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Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells
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September 21, 2010
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The micro-dissected explants technique is a robust and reliable method for isolating proliferative skeletal muscle cells from juvenile, adult or embryonic muscles as a source of skeletal muscle stem cells. Uniquely, these cells have been clonally derived to produce skeletal muscle stem cell lines used for in vivo transplantation.
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Skeletal Muscle Microexplant CultureIsolation Of Skeletal Muscle Stem CellsEmbryonic Skeletal Muscle CellsAdult Skeletal Muscle CellsMicro-dissected Explants TechniqueProliferative Skeletal Muscle CellsGrowth CharacteristicsCell SurvivalProliferationSenescenceDifferentiationExplant Outgrowth RatesPrimary CulturesEmbryonic Skeletal Muscle PrecursorsClonal SMSc LinesIn Vivo TransplantationSatellite CellsSkeletal Muscle Fibre Regeneration
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Merrick, D., Chen, H., Larner, D., Smith, J. Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells. J. Vis. Exp. (43), e2051, doi:10.3791/2051 (2010).
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